The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 16, 2025

Filed:

Jan. 19, 2023
Applicant:

Illumina, Inc., San Diego, CA (US);

Inventors:

Dietrich Dehlinger, San Francisco, CA (US);

Ali Agah, Menlo Park, CA (US);

Tracy Helen Fung, San Francisco, CA (US);

Emrah Kostem, Menlo Park, CA (US);

Craig Hetherington, San Francisco, CA (US);

Assignee:

ILLUMINA, INC., San Diego, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 27/414 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6874 (2018.01); G01N 21/64 (2006.01); G01N 21/76 (2006.01); G06F 18/24 (2023.01); G06V 10/75 (2022.01); G16B 30/00 (2019.01);
U.S. Cl.
CPC ...
G01N 27/4145 (2013.01); B01L 3/502715 (2013.01); C12Q 1/6874 (2013.01); G01N 21/6454 (2013.01); G06F 18/24 (2023.01); G06V 10/751 (2022.01); B01J 2219/00711 (2013.01); B01J 2219/00722 (2013.01); B01L 7/52 (2013.01); B01L 2200/147 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0893 (2013.01); C12Q 1/6869 (2013.01); G01N 2021/6432 (2013.01); G01N 2021/6441 (2013.01); G01N 2021/6471 (2013.01); G01N 2021/6478 (2013.01); G01N 2021/6482 (2013.01); G01N 21/76 (2013.01); G01N 21/763 (2013.01); G01N 2223/40 (2013.01); G01N 2223/413 (2013.01); G05B 2219/37563 (2013.01); G16B 30/00 (2019.02);
Abstract

In one embodiment, a sample surface of a biosensor includes pixel areas and holds a plurality of clusters during a sequence of sampling events such that the clusters are distributed unevenly over the pixel areas. In another embodiment, a biosensor has a sample surface that includes pixel areas and an array of wells overlying the pixel areas, the biosensor including two wells and two clusters per pixel area. The two wells per pixel area include a dominant well and a subordinate well. The dominant well has a larger cross section over the pixel area than the subordinate well. In yet another embodiment, an illumination system is coupled to a biosensor that illuminates the pixel areas with different angles of illumination during a sequence of sampling events, including, for a sampling event, illuminating each of the wells with off-axis illumination to produce asymmetrically illuminated well regions in each of the wells.


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