The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 16, 2025
Filed:
Apr. 03, 2023
Applicant:
The Regents of the University of California, Oakland, CA (US);
Inventor:
Schaked Omer Halperin, Berkeley, CA (US);
Assignee:
The Regents of the University of California, Oakland, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); C12N 9/12 (2006.01); C12N 9/78 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); C12N 9/1252 (2013.01); C12N 9/78 (2013.01); C12N 15/1024 (2013.01); C12N 15/11 (2013.01); C12Y 207/07007 (2013.01); C12Y 305/04004 (2013.01); C12Y 305/04005 (2013.01); C12N 2800/80 (2013.01);
Abstract
The present disclosure provides a fusion polypeptide comprising: a) an enzymatically active RNA-guided endonuclease that introduces a single-stranded break in a target DNA; and b) an error-prone DNA polymerase. The present disclosure provides a system comprising: a) a fusion polypeptide of the present disclosure; and b) a guide RNA. The present disclosure provides a cell comprising a fusion polypeptide of the present disclosure, or a system of the present disclosure. The present disclosure provides a method of mutagenizing a target polynucleotide.