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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12492373 B1

PDFFull Text
Date of Patent:
Dec. 09, 2025

Filed:

Dec. 22, 2021

Production method for nerve tissue

Applicants:

Racthera Co., Ltd., Osaka, JP;

Riken, Wako, JP;

Inventors:

Atsushi Kuwahara, Kobe, JP;

Suguru Yamasaki, Kobe, JP;

Yasushi Hiramine, Kobe, JP;

Yoshiki Sasai, Wako, JP;

Masayo Takahashi, Wako, JP;

Assignees:

RACTHERA CO., LTD., Tokyo, JP;

RIKEN, Wako, JP;

Attorney:

Leydig, Voit & Mayer, Ltd.

Primary Examiner:

James D Schultz

Assistant Examiner:

Nicholas A Humphries

Int. Cl.
CPC ...
C12N 5/0793 (2010.01); A61K 35/12 (2015.01); A61K 35/30 (2015.01); A61L 27/00 (2006.01); A61L 27/38 (2006.01); C12N 5/079 (2010.01); C12N 5/0797 (2010.01); C12Q 1/02 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/062 (2013.01); A61K 35/12 (2013.01); A61K 35/30 (2013.01); A61L 27/00 (2013.01); A61L 27/383 (2013.01); A61L 27/3895 (2013.01); C12N 5/0618 (2013.01); C12N 5/0619 (2013.01); C12N 5/0621 (2013.01); C12N 5/0623 (2013.01); C12Q 1/02 (2013.01); G01N 33/5014 (2013.01); C12N 2500/99 (2013.01); C12N 2501/115 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/40 (2013.01); C12N 2501/41 (2013.01); C12N 2501/415 (2013.01); C12N 2501/727 (2013.01); C12N 2506/45 (2013.01); C12N 2533/52 (2013.01);
Abstract

The present invention provides a method for producing neural cells or a neural tissue, including the following steps (1)-(3): (1) a first step of culturing pluripotent stem cells in the absence of feeder cells and in a medium containing 1) a TGFβ family signal transduction pathway inhibiting substance and/or a Sonic hedgehog signal transduction pathway activating substance, and 2) a factor for maintaining undifferentiated state,(2) a second step of culturing the cells obtained in the first step in suspension to form a cell aggregate, and(3) a third step of culturing the aggregate obtained in the second step in suspension in the presence or absence of a differentiation-inducing factor to obtain an aggregate containing neural cells or a neural tissue.

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