The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 02, 2025

Filed:

Jan. 11, 2022
Applicant:

Genzyme Corporation, Cambridge, MA (US);

Inventors:

Christopher Hwang, Cambridge, MA (US);

Timothy Johnson, Cambridge, MA (US);

Jason Walther, Cambridge, MA (US);

Cheng Cheng, Cambridge, MA (US);

Jonathan Wang, Cambridge, MA (US);

Neha Shah, Cambridge, MA (US);

Seul-A Bae, Cambridge, MA (US);

Assignee:

Genzyme Corporation, Cambridge, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/00 (2006.01); C07K 16/00 (2006.01); C12N 5/00 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C07K 16/00 (2013.01); C12N 5/0043 (2013.01); C12N 5/005 (2013.01); C07K 2317/14 (2013.01); C12N 2500/50 (2013.01); C12N 2510/02 (2013.01);
Abstract

Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.


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