The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 25, 2025

Filed:

Feb. 12, 2021
Applicant:

Arizona Board of Regents on Behalf of Arizona State University, Scottsdale, AZ (US);

Inventors:

Nongjian Tao, Scottsdale, AZ (US);

Shaopeng Wang, Chandler, AZ (US);

Pengfei Zhang, Scottsdale, AZ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01); B01L 3/00 (2006.01); G01N 15/1434 (2024.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6803 (2013.01); B01L 3/502761 (2013.01); G01N 15/1434 (2013.01); G01N 33/54386 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/12 (2013.01);
Abstract

Detecting single molecules includes binding the single molecules to a surface of an optically transparent substrate, irradiating the surface of the substrate with light having an incident angle selected to achieve total reflection of the light, thereby scattering light from the surface and from the single molecules bound to the surface, and collecting light scatted by the surface and by the single molecules bound to the surface to form a series of images. Systems for detecting single molecules include an optically transparent substrate, a means for flowing a sample solution over a surface of the substrate, a light source configured to irradiate the surface, a camera, and a collection optical system configured to collect light scatted by the surface and by the target molecules on the surface to form a series of images on the camera.


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