The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 18, 2025

Filed:

May. 14, 2020
Applicant:

Institut Pasteur, Paris, FR;

Inventors:

Giulia Manina, Paris, FR;

Maxime Mistretta, Paris, FR;

Assignee:

INSTITUT PASTEUR, Paris, FR;

Attorney:
Int. Cl.
CPC ...
C12Q 1/18 (2006.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12N 1/20 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/18 (2013.01); B01L 3/502715 (2013.01); C12M 23/22 (2013.01); C12M 23/34 (2013.01); C12M 41/36 (2013.01); C12N 1/20 (2013.01); B01L 2200/0684 (2013.01); B01L 2300/0654 (2013.01); B01L 2400/0655 (2013.01);
Abstract

Multiplexable microfluidic culture chamber for imaging monolayer growth of single cells The present invention relates generally to a microfluidic device (), particularly for use in single cell analysis. More specifically, the present invention relates to a microfluidic device () comprising at least one chamber (), in particular at least two chambers () comprising a deformable membrane () and having a structure and geometry configured to enable formation of two-dimensional cell culture, in particular two-dimensional cell growth area (), and imaging thereof over a growth period or a time period sufficient to analyze cells, in particular to monitor cell growth. The microfluidic device () allows for multi-condition operation of single-cell screening at high spatiotemporal resolution. The present invention also relates to methods for fabrication and use of such devices.


Find Patent Forward Citations

Loading…