The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 18, 2025

Filed:

Feb. 01, 2019
Applicant:

The General Hospital Corporation, Boston, MA (US);

Inventors:

Osman Berk Usta, Swampscott, MA (US);

Haishui Huang, Allston, MA (US);

Martin Yarmush, Newton, MA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/00 (2006.01); A01N 1/125 (2025.01); B01F 23/41 (2022.01); B01F 23/80 (2022.01); C12N 1/04 (2006.01); C12N 1/30 (2006.01); C12N 5/00 (2006.01); B01F 23/40 (2022.01);
U.S. Cl.
CPC ...
B01F 23/802 (2022.01); A01N 1/125 (2025.01); B01F 23/4105 (2022.01); B01F 23/48 (2022.01);
Abstract

This disclosure relates to methods of super-cooling of aqueous samples with or without biological samples. The methods involve, e.g., providing a container comprising the aqueous sample; applying an immiscible liquid phase of sufficient thickness to separate the aqueous sample from air; and cooling the aqueous sample to a temperature that is below 0° C.


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