The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 11, 2025
Filed:
Oct. 03, 2022
Qingdao Agricultural University, Qingdao, CN;
Qingdao Agricultural University, Qingdao, CN;
Abstract
Provided is a method for continuous detection of a miRNA by a metal-organic framework (MOF)-based nanozyme, including the following steps: step 1, conducting a duplex-specific nuclease (DSN)-assisted signal amplification strategy; and step 2, conducting the flow analysis on a target using a flow injection-batch method, and recording a differential pulse voltammetry (DPV) response of a biosensor in a batch mode; and step 3, in the flow mode, conducting competitive elution on a MOF/indium tin oxide (ITO) electrode with a complementary DNA (cDNA) as a mobile phase. In the present invention, a two-dimensional MOF-based nanozyme is simply synthesized by an ultrasonic method and then applied to a homogeneous electrochemical biosensor without a complicated electrode modification process, showing a continuous detection function, a high selectivity and a desirable sensitivity.