The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 11, 2025
Filed:
Nov. 18, 2019
Applicant:
Altius Institute for Biomedical Sciences, Seattle, WA (US);
Inventors:
John Stamatoyannopoulos, Seattle, WA (US);
Pavel Zrazhevskiy, Seattle, WA (US);
Assignee:
Altius Institute for Biomedical Sciences, Seattle, WA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6816 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6816 (2013.01);
Abstract
Provided herein are methods and compositions for use in detecting on-target and/or off-target cleavage of genomic DNA in a cell by a nuclease. The on-target and off-target cleavage is detected by using oligonucleotides that bind to the single-stranded 3'-overhang created at the cleavage site. The nuclease may be an engineered nuclease comprising a nucleic acid binding domain that binds to a target nucleic acid of interest.