The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 04, 2025

Filed:

May. 15, 2019
Applicants:

Alliance for Sustainable Energy, Llc, Golden, CO (US);

Ut-battelle, Llc, Oak Ridge, TN (US);

Inventors:

Gregg Tyler Beckham, Golden, CO (US);

Thelhawadigedara Lahiru Niroshan Jayakody, Wheat Ridge, CO (US);

Adam Michael Guss, Oak Ridge, TN (US);

Thomas David Mand, Oak Ridge, TN (US);

Christopher W. Johnson, Denver, CO (US);

Isabel Pardo Mendoza, Dos Hermanas, ES;

Assignees:

Alliance for Sustainable Energy, LLC, Golden, CO (US);

UT-Battelle, LLC, Oak Ridge, TN (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/52 (2006.01); C12N 1/20 (2006.01); C12N 9/18 (2006.01);
U.S. Cl.
CPC ...
C12N 1/20 (2013.01); C12N 9/18 (2013.01); C12Y 301/01 (2013.01);
Abstract

Disclosed herein are engineeredKT2440 co-expressing PETase and MHETase enzymes that selectively degrades PET into monomers, ethylene glycol and terephthalate (TPA). In another embodiment, disclosed herein are methods for making and using a highly efficient EG metabolizingKT2440 strain. Given that nativedoes not have a TPA metabolic pathway, nor the proteins to transport TPA into the cell, the next metabolic engineering challenge for developing syntheticstrain to plastic upcycling was enabling TPA catabolisminKT2440. TPA transporters and catabolic pathway have been characterized in several microorganisms includingsp. strain E6 andRHA1.


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