The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 04, 2025

Filed:

Oct. 28, 2022
Applicant:

Nextern Innovation, Llc, White Bear Lake, MN (US);

Inventors:

Jon F. Urban, Minneapolis, MN (US);

Riazul Islam, St. Paul, MN (US);

John Swoyer, Blaine, MN (US);

Fernando Buarque, White Bear Lake, MN (US);

John D. Foley, Libertyville, IL (US);

Assignee:

Nextern Innovation, LLC, White Bear Lake, MN (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/04 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12M 3/00 (2006.01);
U.S. Cl.
CPC ...
C12M 23/24 (2013.01); C12M 23/40 (2013.01); C12M 23/44 (2013.01); C12M 23/54 (2013.01); C12M 25/06 (2013.01); C12M 29/04 (2013.01); C12M 41/34 (2013.01); C12M 41/48 (2013.01);
Abstract

Apparatus and associated methods relate to cell culture systems having controlled gas concentration boundary conditions around cell culture. In an illustrative example, a programmable cell culture system (PCCS) may include one or more manifolds, each releasably coupled to multiple cell culture modules. The cell culture modules, for example, may include a nutrient capacitive medium (NCM) in fluid communication with a concentration-controlled gas source through at least two gas transfer interfaces. The gas transfer interfaces (GTIs) may, for example, be spatially distributed in relation to a cell growth surface in the NCM. Each GTI, for example, may include a gas permeable membrane (GPM) and a plenum. The GPM may be disposed between the plenum and the NCM. Various embodiments may advantageously selectively control a concentration of at least one target gas in the NCM by maintaining predetermined boundary conditions of the cell growth surface via the GTIs.


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