The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 04, 2025

Filed:

Oct. 13, 2021
Applicants:

George Mason Research Foundation, Inc., Fairfax, VA (US);

The Trustees of the University of Pennsylvania, Philadelphia, PA (US);

Inventors:

Michael Daro Buschmann, Fairfax, VA (US);

Mikell Paige, Fairfax, VA (US);

Suman Alishetty, Fairfax, VA (US);

Manuel Carrasco, Fairfax, VA (US);

Mohamad Gabriel Alameh, Fairfax, VA (US);

Drew Weissman, Fairfax, VA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61K 9/51 (2006.01); A61K 9/127 (2006.01); A61K 31/7088 (2006.01); A61K 31/7105 (2006.01); A61K 47/18 (2017.01); A61K 47/28 (2006.01); A61P 37/04 (2006.01); B82Y 5/00 (2011.01); C07C 229/16 (2006.01); C07D 207/09 (2006.01); C07D 211/26 (2006.01); C07D 295/13 (2006.01); C12N 15/88 (2006.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
A61K 9/5123 (2013.01); A61K 9/127 (2013.01); A61K 31/7088 (2013.01); A61K 31/7105 (2013.01); A61K 47/18 (2013.01); A61K 47/28 (2013.01); A61P 37/04 (2018.01); B82Y 5/00 (2013.01); C07C 229/16 (2013.01); C07D 207/09 (2013.01); C07D 211/26 (2013.01); C07D 295/13 (2013.01); C12N 15/88 (2013.01); A61K 48/00 (2013.01);
Abstract

Disclosed herein are methods of increasing the potency of nucleic acid loaded lipid nanoparticles (naLNPs) through certain novel and surprisingly superior LNP manufacturing techniques. Also disclosed are pharmaceutical compositions containing naLNPs manufactured according to the manufacturing methods described herein. The methods disclosed herein overcome major technical difficulties and high costs associated with previous LNP manufacturing techniques. The methods disclosed herein, therefore, greatly improve the industrial production of LNPs in unexpected ways thereby providing more potent naLNPs for nucleic acid delivery. Specifically, the invention disclosed herein are methods that show increased potency naLNPs due to increased mixing concentration of the lipids and mRNA during assembly.


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