The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 28, 2025

Filed:

Apr. 08, 2021
Applicant:

Takara Bio Usa, Inc., Mountain View, CA (US);

Inventors:

Craig Betts, Mountain View, CA (US);

Andrew Alan Farmer, Los Altos, CA (US);

Nathalie Bolduc, Mountain View, CA (US);

Assignee:

Takara Bio USA, Inc., San Jose, CA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12Q 1/6853 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6853 (2013.01); C12N 15/1096 (2013.01); C12P 19/34 (2013.01); C12Y 207/07007 (2013.01); C12Y 301/00 (2013.01); C12Q 2521/107 (2013.01); C12Q 2525/191 (2013.01);
Abstract

Provided are methods of producing a product nucleic acid. The methods include combining a template deoxyribonucleic acid (DNA), a polymerase, a template switch oligonucleotide, and dNTPs into a reaction mixture. The components are combined into the reaction mixture under conditions sufficient to produce a product nucleic acid that includes the template DNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.


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