The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 28, 2025
Filed:
Jul. 13, 2021
Applicant:
Illumina, Inc., San Diego, CA (US);
Inventors:
Fiona Kaper, San Diego, CA (US);
Gordon Cann, Hayward, CA (US);
Assignee:
Illumina, Inc., San Diego, CA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1065 (2013.01); C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01); C12N 15/1003 (2013.01);
Abstract
A method of preparing a library of tagged nucleic acid fragments including contacting a population of cells directly with a lysis reagent having one or more protease to generate a cell lysate; inactivating the protease to generate an inactivated cell lysate, and applying a transposase and a transposon end composition containing a transferred strand to the inactivated cell lysate under conditions wherein the target nucleic acid and the transposon end composition undergo a transposition reaction.