The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 21, 2025
Filed:
Aug. 16, 2023
Nanjing Drum Tower Hospital, Jiangsu, CN;
Can Xu, Jiangsu, CN;
Dongjin Wang, Jiangsu, CN;
NANJING DRUM TOWER HOSPITAL, Jiangsu, CN;
Abstract
The present invention discloses a staged culture method for in-vitro expansion of human CIK cells and use thereof. In order to solve the problem of increase of a proportion of necrotic cells in CIK cells caused by adding exogenous IL-12 in the prior art, time of IL-12 induction is shortened by twice induction in stages. A primary induction culture medium containing IL-2, IL-7 and the IL-12 is used for primary induction and a re-induction culture medium containing the IL-2, the IL-7 and a CD28 antibody is used for re-induction. The result shows that the proportion of the necrotic cells in the CIK cells obtained by the staged twice induction method is obviously lower than that obtained by a continuous induction culture method commonly used in the prior art. Besides, a necrosis rate is influenced by primary induction time. When the primary induction time is 60 h, the necrosis rate is 1.02% at the lowest, which is reduced by 69.7% compared with that of a control group. The CIK cells obtained by the present application have a low necrosis rate and high activity, and can be widely used in the treatment of tumors.