The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 21, 2025
Filed:
Nov. 24, 2024
Jitri Future Food Technology Institute Co., Ltd., Jiangsu, CN;
Jiangnan University, Jiangsu, CN;
Xiaomei Zhang, Wuxi, CN;
Yamin Huang, Wuxi, CN;
Yujie Gao, Wuxi, CN;
Guoqiang Xu, Wuxi, CN;
Hui Li, Wuxi, CN;
Jinsong Shi, Wuxi, CN;
Zhenghong Xu, Wuxi, CN;
Jitri Future Food Technology Institute Co., Ltd., Wuxi, CN;
Jiangnan University, Wuxi, CN;
Abstract
The present invention provides a transcription factor NCgl0581 mutant and use thereof in L-serine detection, belonging to the technical field of biological detection. According to the present invention, key binding sites of the transcription factor NCgl0581 with L-serine are obtained through molecular docking, and further model analysis and virtual screening are carried out to obtain an L-serine biosensor mutant NCgl0581. Experiments show that the biosensor constructed based on the mutant NCgl0581can respond to 25 mM serine, which is half of the response concentration before the mutation. The present invention reduces the detection limit of the biosensor through mutation, lays a foundation for the detection of low-concentration L-serine, and has good application value and prospect.