The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 21, 2025

Filed:

May. 28, 2021
Applicant:

Kimberly-clark Worldwide, Inc., Neenah, WI (US);

Inventor:

Stephen Quirk, Alpharetta, GA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B09B 3/60 (2022.01); B09B 101/67 (2022.01); B09B 101/75 (2022.01); C12N 9/18 (2006.01);
U.S. Cl.
CPC ...
B09B 3/60 (2022.01); C12N 9/18 (2013.01); C12Y 301/01075 (2013.01); B09B 2101/67 (2022.01); B09B 2101/75 (2022.01);
Abstract

The present invention relates to a method for treatment of polyhydroxyalkanoate (PHA) containing post-consumer product, the method comprising contacting a post-consumer product with a polypeptide that can catalyze degradation of the PHA, the contact taking place at a temperature at least 45° C. In a specific embodiment, the poly peptide is a wild-type PHA depolymerase expressed by a thermophilic microorganism or a modified PHA depolymerase that includes one or more single-site mutations as compared to the wild-type PHA depolymerase. In another specific embodiment, the polypeptide comprising a modified poly hydroxy butyrate (PHB) depolymerase comprising one or more single-site mutations as compared to SEQ ID NO: 1, and the modified PHB depolymerase having an optimum temperature of at least 45° C. The present invention also relates to a host cell transformed to express a polypeptide that catalyzes degradation of a PHA, the polypeptide having an optimum temperature for the degradation reaction of at least 45° C., wherein the host cell is selected from ancell or a thermophilic microorganism.


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