Extracellular matrix hydrogel microsphere, and preparation method and application thereof

Abstract

The present disclosure relates to the field of biomaterial technology, and discloses an extracellular matrix hydrogel microsphere and a preparation method thereof. The method includes: constructing a neonatal rat bone marrow mesenchymal stem cell-derived extracellular matrix; preparing hydrogel microspheres by microfluidic technique; and using a condensing agent DMTMM to activate carboxyl groups on surfaces of hydrogel microspheres, and making the activated hydrogel microspheres react with amino groups of the extracellular matrix to obtain extracellular matrix hydrogel microspheres. The present disclosure constructs an extracellular matrix hydrogel microsphere, which realizes the advantage complementary between a hydrogel and a stem cell-derived extracellular matrix, and retains the biocompatibility of a hydrogel precursor material while having mechanical properties. By combining a hydrogel microsphere with an extracellular matrix, bone formation of bone marrow mesenchymal stem cells in vitro may be significantly promoted, and osteoclast differentiation is inhibited, which provides more possibilities for cranial defect repair.