The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 07, 2025
Filed:
Aug. 15, 2022
Applicant:
10x Genomics, Inc., Pleasanton, CA (US);
Inventor:
Malte Kühnemund, Stockholm, SE;
Assignee:
10x Genomics, Inc., Pleasanton, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6841 (2018.01); C12Q 1/6827 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6851 (2018.01); C12Q 1/6876 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6841 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6876 (2013.01); C12Q 1/6851 (2013.01);
Abstract
In some aspects, the present disclosure relates to methods for reducing the detection of false positive ligation events. In some aspects, the method comprises use of a double split (or 'split split') probe. The methods herein have particular applicability in reducing the detection of false positive ligation events when using ligases that have high ligation efficiency but low specificity (e.g., SplintR® ligase). Also provided are kits comprising probes for use in such methods.