The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 07, 2025

Filed:

Jan. 11, 2019
Applicant:

Stem Arts Projects, Llc, Lincoln, NE (US);

Inventors:

Abraham Oommen, Lincoln, NE (US);

Heather Piscatelli, Lincoln, NE (US);

Alyssa Hangman, Lincoln, NE (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6827 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6827 (2013.01);
Abstract

Reaction condition compositions for detecting a genomic variation from a small sample amount from 5 nano grams (ng) to 1 microgram (ug) includes DNA ligase, DNA polymerase, at least one COP, a DNA polymerase buffer, NAD+, at least two primers, and deoxynucleotide triphosphates (dNTPs). Detection of the genomic variation utilizes COPs with increased ligation efficiency and RCA with fluorescence detection due to simultaneous ligation of COPs to CPs and replication of the genomic variation. The reaction condition composition eliminates the need to perform background reduction of un-hybridized or un-ligated COPs.


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