The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 07, 2025
Filed:
May. 26, 2023
Applicant:
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Inventors:
Sedide Ozturk, Pleasanton, CA (US);
Martin Ranik, Santa Clara, CA (US);
Florian Rubelt, Menlo Park, CA (US);
Assignee:
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6853 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6876 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6809 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6876 (2013.01); C12Q 2521/101 (2013.01); C12Q 2521/107 (2013.01); C12Q 2521/327 (2013.01); C12Q 2525/117 (2013.01); C12Q 2525/155 (2013.01); C12Q 2525/161 (2013.01); C12Q 2525/173 (2013.01); C12Q 2531/10 (2013.01); C12Q 2537/149 (2013.01); C12Q 2563/179 (2013.01); C12Q 2563/185 (2013.01);
Abstract
The invention is a method of single cell transcriptome analysis. The method comprises detecting multiple transcripts in each individual cell of the plurality of cells by barcoding the transcripts with a cell-specific compound barcode formed using a DNA polymerase and a terminal transferase, optionally in a single enzyme such as a reverse transcriptase.