The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 07, 2025

Filed:

Jun. 10, 2011
Applicants:

Markus Kostrzewa, Lilienthal, DE;

Karsten Michelmann, Bremen, DE;

Katrin Sparbier, Bremen, DE;

Inventors:

Markus Kostrzewa, Lilienthal, DE;

Karsten Michelmann, Bremen, DE;

Katrin Sparbier, Bremen, DE;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/18 (2006.01); C12Q 1/34 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/18 (2013.01); C12Q 1/34 (2013.01); G01N 33/6851 (2013.01); G01N 2333/986 (2013.01); G01N 2800/26 (2013.01); G01N 2800/44 (2013.01);
Abstract

The invention relates to the determination of resistances of microorganisms which produce β-lactamases, in particular 'extended spectrum β-lactamases' (ESBL). The invention provides a method whereby the microbial resistance can be measured very simply and quickly by means of the catalytic effect of the microbially produced β-lactamases on β-lactam antibiotics, which consists in a hydrolytic cleavage of the β-lactam ring. The method determines the resistance of the bacteria a few hours after a suitable substrate, either a β-lactam antibiotic or a customized β-lactam derivative, has been added to a suspension of the microbes, by direct mass spectrometric measurement of the substrate breakdown caused by the β-lactamases.


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