The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 07, 2025
Filed:
Jul. 29, 2024
Shanghai Hongene Biotech Corporation, Shanghai, CN;
Shanghai Hongene Bioengineering Co., Ltd, Shanghai, CN;
SHANGHAI HONGENE BIOTECH CORPORATION, Shanghai, CN;
SHANGHAI HONGENE BIOENGINEERING CO., LTD, Shanghai, CN;
Abstract
The present disclosure provides a complex for synthesizing one or more nucleotide strands of interest and a method for synthesizing one or more nucleotide strands of interest. The complex for synthesizing one or more nucleotide strands of interest includes nucleotide fragments and a double-stranded oligonucleotide ligase, wherein the nucleotide fragments are hybridized to form a double-stranded oligonucleotide; the double-stranded oligonucleotide ligase is a ligase capable of sealing nicks in the double-stranded oligonucleotide; and at least one strand of the double-stranded oligonucleotide is a nucleotide strand of interest; and the nucleotide fragments include a first fragment and a second fragment, the first fragment contains a 5'-phosphate group, and the first fragment is synthesized in a 3′- to 5′-direction; and the second fragment contains a 3′-hydroxyl group, and the second fragment is synthesized in a 5′- to 3′-direction.