The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 30, 2025
Filed:
May. 18, 2018
Applicant:
Toyota Jidosha Kabushiki Kaisha, Toyota, JP;
Inventors:
Assignee:
TOYOTA JIDOSHA KABUSHIKI KAISHA, Toyota, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6895 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6895 (2013.01); C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01);
Abstract
When preparing a DNA library via a nucleic acid amplification reaction using a random primer in a convenient and highly reproducible manner, amplification of DNA fragments derived from the chloroplast genome is reduced to a significant extent. A random primer comprises oligonucleotides selected from oligonucleotides group represented by TAAGAGACAGNN excluding those in which 2 bases at the 3' terminus are TG and oligonucleotides group represented by TAAGAGACAGNNN excluding those in which 3 bases at the 3′ terminus are TGC.