The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 30, 2025
Filed:
Aug. 06, 2019
Genemind Biosciences Company Limited, Shenzhen, CN;
Guangli Gan, Shenzhen, CN;
Qunting Lin, Shenzhen, CN;
Yunyang Cheng, Shenzhen, CN;
Meng Zhang, Shenzhen, CN;
Gailing Li, Shenzhen, CN;
Qin Yan, Shenzhen, CN;
Luyang Zhao, Shenzhen, CN;
GeneMind Biosciences Company Limited, Shenzhen, CN;
Abstract
Disclosed are a method for ligating nucleic acid fragments, a method for constructing a sequencing library, and the use thereof. The method for ligating nucleic acid fragments comprises ligating a first nucleic acid fragment and a second nucleic acid fragment in a mixed enzyme reaction system comprising a DNA ligase and a RNA ligase, wherein the first nucleic acid fragment is double-stranded DNA. The ligation efficiency of the method for ligating nucleic acid fragments can reach 85% or more, and by using this ligation method to construct a sequencing library, the yield of the effective library is significantly improved.