The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 23, 2025
Filed:
Jan. 09, 2025
Shenzhen Blood Center, Guangdong, CN;
Shuang Liang, Guangdong, CN;
Jinfeng Zeng, Guangdong, CN;
Yanlian Liang, Guangdong, CN;
Fan Wu, Guangdong, CN;
Tong Liu, Guangdong, CN;
Liyan Sun, Guangdong, CN;
Shenzhen Blood Center, Guangdong, CN;
Abstract
A method and a kit for genotyping of multi-system red blood cell (RBC) blood group based on next-generation sequencing (NGS) are provided. Multiplex PCR capture primer sets and amplification reaction conditions thereof can be designed using Illumina as a platform to simultaneously detect full-length coding regions and spliced flanking regions of 25 blood group genes in 17 RBC blood group systems among three reactions, thereby enabling rapid genotyping of the 17 RBC blood group systems and multiple key rare blood groups therein. The method and the kit are based on high-throughput sequencing and have a high detection efficiency and a low average cost; the method and the kit also break through a limitation of existing blood group gene detection technology that cannot distinguish haplotypes; moreover, variations including known mutation sites and unknown mutation sites can be detected to obtain comprehensive and accurate detection results.