The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 23, 2025

Filed:

Feb. 01, 2024
Applicant:

Translate Bio, Inc., Lexington, MA (US);

Inventors:

Frank Derosa, Lexington, MA (US);

Anusha Dias, Lexington, MA (US);

Michael Heartlein, Lexington, MA (US);

Assignee:

TRANSLATE BIO, INC., Lexington, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6804 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6804 (2013.01); C12Q 1/6804 (2013.01); C12Q 2522/101 (2013.01); C12Q 2525/186 (2013.01); C12Q 2537/125 (2013.01); C12Q 2563/125 (2013.01);
Abstract

The present invention provides, among other things, methods of quantitating mRNA capping efficiency, particularly mRNA synthesized in vitro. In some embodiments, methods according to the present invention comprise providing an mRNA sample containing capped and uncapped mRNA, providing a cap specific binding substance under conditions that permit the formation of a complex between the cap specific binding substance and the capped mRNA, and quantitatively determining the amount of the complex as compared to a control, thereby quantifying mRNA capping efficiency.


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