The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 23, 2025
Filed:
Nov. 27, 2019
Applicant:
Nanjing Nutrabuilding Bio-tech Co., Ltd., Nanjing, CN;
Inventors:
Qilin Liao, Nanjing, CN;
Jian Zhang, Nanjing, CN;
Assignee:
NANJING NUTRABUILDING BIO-TECH CO., LTD., Nanjing, CN;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 13/04 (2006.01); C12N 9/10 (2006.01); C12N 15/70 (2006.01);
U.S. Cl.
CPC ...
C12P 13/04 (2013.01); C12N 9/13 (2013.01); C12N 15/70 (2013.01); C12N 2800/101 (2013.01);
Abstract
Provided herein includes a method for producing ergothioneine, comprising N(α)-trimethyl histidine and an oxidative sulfurizing enzyme mutant. With the mutant enzyme's help, the conversion rate is higher than 30% with the mutant enzyme amount of 8000/g substrate in 24 hours. Disclosed are a nucleic acid encoding the mutant enzyme, an expression vector comprising the nucleic acid, an expressing host comprising the nucleic acid or the expression vector, and the use of the mutant enzyme EanB for producing the ergothioneine.