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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12419340 B1

PDFFull Text
Date of Patent:
Sep. 23, 2025

Filed:

Sep. 26, 2022

Microbial fermentation method for improving tobacco quality

Applicant:

Yunnan Academy of Tobacco Agricultural Sciences, Kunming, CN;

Inventors:

Binbin Hu, Kunming, CN;

Jing Mai, Kunming, CN;

Mingjun Zhu, Kunming, CN;

Congming Zou, Kunming, CN;

Zhonglong Lin, Kunming, CN;

Ying Ning, Kunming, CN;

Yi Chen, Kunming, CN;

Yonglei Jiang, Kunming, CN;

Ling Zou, Kunming, CN;

Guiyan Zhou, Kunming, CN;

Baoxing Wang, Kunming, CN;

Jieyun Cai, Kunming, CN;

Haowei Sun, Kunming, CN;

Ke Zhang, Kunming, CN;

Xiaowei Zhang, Kunming, CN;

Assignee:

Yunnan Academy of Tobacco Agricultural Sciences, Kunming, CN;

Attorney:

Nitin Kaushik

Primary Examiner:

Philip Y Louie

Assistant Examiner:

Yared Tarekegn Kokeb

Int. Cl.
CPC ...
A24B 15/20 (2006.01); C12N 1/20 (2006.01); C12P 1/04 (2006.01);
U.S. Cl.
CPC ...
A24B 15/20 (2013.01); C12N 1/20 (2013.01); C12P 1/04 (2013.01);
Abstract

A microbial fermentation method for improving tobacco quality, comprising the following steps: (1) inoculating flue-cured tobacco as an inoculation source into a tobacco powder medium, and cultivating at 20-40° C. for 20-50 h; (2) inoculating microbial liquid in step (1) into a fresh tobacco powder medium, cultivating at 20-40° C. for 20-50 h, and circulating for 15-25 cycles to obtain stable flue-cured tobacco microbial flora; (3) inoculating the microbial flora obtained in step (2) into the fresh tobacco powder medium, and culturing at 20-40° C. for 20-50 h to obtain seed liquid of the microbial flora; (4) centrifuging the microbial liquid obtained in step (3) and collecting microbes; (5) washing the microbes collected in step (4); (6) resuspending the washed bacteria in step (5) to obtain a microbial suspension; and (7) spraying the microbial suspension in step (6) to the tobacco leaves for fermentation for 6-8 days.

Find Patent Forward Citations

Hu, Binbin. (2025). Microbial fermentation method for improving tobacco quality (U.S. Patent No. 12419340). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/12419340

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