The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 23, 2025

Filed:

Nov. 29, 2023
Applicant:

Monsanto Technology Llc, St. Louis, MO (US);

Inventors:

Ying Guo, Ballwin, MO (US);

Daniel Jefferson Karcher, St. Louis, MO (US);

Huachun Wang Larue, Chesterfield, MO (US);

Huai Wang, Chesterfield, MO (US);

Assignee:

Monsanto Technology LLC, St. Louis, MO (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A01H 4/00 (2006.01); A01G 2/00 (2018.01); A01G 24/22 (2018.01);
U.S. Cl.
CPC ...
A01H 4/005 (2013.01); A01G 2/00 (2018.02); A01G 24/22 (2018.02);
Abstract

The present disclosure provides novel methods and compositions for the maturation and regeneration of plantlets from microspore-derived embryos. The compositions provided include a liquid maturation composition comprising a first plant hormone and about 430 mM to about 880 mM of a monosaccharide solute, a disaccharide solute, or a polysaccharide solute. The methods provided include the steps of contacting a microspore-derived embryo with a liquid maturation medium for a time period sufficient to produce a matured microspore-derived embryo, transferring the matured microspore-derived embryo to a substrate, and regenerating the plantlet from the matured microspore-derived embryo.


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