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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12416052 B1

PDFFull Text
Date of Patent:
Sep. 16, 2025

Filed:

Jun. 24, 2024

Capturing, concentrating, and detecting microbes in a sample using magnetic ionic liquids and recombinase polymerase amplification

Applicant:

Iowa State University Research Foundation, Inc., Ames, IA (US);

Inventors:

Jared L. Anderson, Ames, IA (US);

Byron F. Brehm-Stecher, Ames, IA (US);

Kevin D. Clark, Ames, IA (US);

Stephanie A Hice, Ames, IA (US);

Assignee:

Iowa State University Research Foundation, Inc., Ames, IA (US);

Attorney:

McKee, Voorhees & Sease, PLC

Primary Examiner:

Jezia Riley

Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C07F 5/00 (2006.01); C07F 13/00 (2006.01); C07F 15/04 (2006.01); C07F 15/06 (2006.01); C12N 1/02 (2006.01); C12N 1/20 (2006.01); C12N 15/10 (2006.01); C12Q 1/04 (2006.01); C12Q 1/24 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/689 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/689 (2013.01); C07F 5/003 (2013.01); C07F 13/005 (2013.01); C07F 15/045 (2013.01); C07F 15/065 (2013.01); C12N 1/02 (2013.01); C12N 1/20 (2013.01); C12N 15/1013 (2013.01); C12Q 1/04 (2013.01); C12Q 1/24 (2013.01); C12Q 1/6806 (2013.01);
Abstract

In this disclosure, a method or kit for using the method of extracting, concentrating, and detecting microbes from a sample is disclosed. The method disclosed herein use transition or rare earth metal-based magnetic ionic liquids (MILs) to extract viable microbes from a sample and to detect them via an amplification-based method and/or a non-amplification-based methods. The method and kit can be used in-field and on-site for detection of viable microbes in a sample within about an hour, without using any powered heat source or powered tool.

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