The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 16, 2025

Filed:

Nov. 25, 2020
Applicants:

Illumina, Inc., San Diego, CA (US);

Illumina Cambridge Limited, Cambridge, GB;

Inventors:

Wayne N. George, Ilford, GB;

Brian D. Mather, San Diego, CA (US);

Andrew A. Brown, Cambridge, GB;

Pietro Gatti Lafranconi, Cambridge, GB;

Maria Candelaria Rogert Bacigalupo, Encinitas, CA (US);

Antoine Francais, Cambridge, GB;

Xiaohai Liu, Cambridge, GB;

Assignees:

Illumina, Inc., San Diego, CA (US);

Illumina Cambridge Limited, Cambridge, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6848 (2013.01); C12Q 1/6869 (2013.01);
Abstract

An example of a kit includes a flow cell and a cleavage mix. An example flow cell includes a substrate; a catalytic polymeric hydrogel on the substrate, the catalytic polymeric hydrogel including a deblocking catalyst; and an amplification primer attached to the catalytic polymeric hydrogel. The deblocking catalyst accelerates cleavage of a blocking group of a 3' OH blocked nucleotide introduced to the flow cell and incorporated into a template strand attached to the amplification primer. An example of the cleavage mix includes a component to initiate cleavage of the blocking group.


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