The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 09, 2025
Filed:
Mar. 18, 2025
Jiangsu University, Zhenjiang, CN;
Jiangsu University, Zhenjiang, CN;
Abstract
A primer set ofbased on specific hyperbranched rolling circle amplification (HRCA) isothermal amplification includes: Lock and Key series primers for a recognition stage, and Loop and Link primers for an amplification stage. Using the primer set for an HRCA reaction enables rapid and sensitive detection with reliable and stable results. A method for detecting theincludes: (1) constructing a 'lock' structure with recognition function; (2) performing magnetic selection and recognition; (3) conducting HRCA amplification; and (4) identifying a fluorescent signal to determine a positive or negative result determination. The method adopts the “lock” structure to design multiple conserved fragments of theinsertion sequence 6110 (IS6110) as target genes to jointly trigger the HRCA reaction, thereby enhancing the accuracy of the method.