The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 09, 2025

Filed:

Oct. 23, 2023
Applicant:

Watchmaker Genomics, Inc., Boulder, CO (US);

Inventors:

Martin Ranik, Boulder, CO (US);

Eric Van Der Walt, Cape Town, ZA;

Clara Ross, Lafayette, CO (US);

Craig Marshall, Boulder, CO (US);

Lindsay Peterkin, Longmont, CO (US);

Brian Kudlow, Boulder, CO (US);

Travis J. Sanders, Boulder, CO (US);

Assignee:

Watchmaker Genomics, Inc., Boulder, CO (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/6834 (2018.01); C12N 9/22 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6834 (2013.01); C12N 9/22 (2013.01); C12N 2310/20 (2017.05); C12Q 2600/166 (2013.01);
Abstract

Disclosed herein are methods and compositions for normalizing polynucleotide concentration. Normalizing may be accomplished using polynucleotide binding proteins (e.g., catalytically inactive CRISPR protein or catalytically inactive Argonaute proteins). The polynucleotide binding proteins may bind to the adapter sequences of a target polynucleotide. Thus, adding the same amount of a polynucleotide binding proteins to different samples and then extracting the polynucleotide protein is shown herein to extract similar amounts of target polynucleotides from the different samples.


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