The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 09, 2025

Filed:

Jul. 12, 2022
Applicants:

C-lecta Gmbh, Leipzig, DE;

New Matterhorn, Llc, Wilmington, DE (US);

Inventors:

Christopher David Bayer, Amsterdam, NL;

Andreas Vogel, Leipzig, DE;

Marc Struhalla, Leipzig, DE;

Birgit Brucher, Leipzig, DE;

Assignees:

C-LECTA GMBH, Leipzig, DE;

NEW MATTERHORN, LLC, Wilmington, DE (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/10 (2006.01); C12N 9/92 (2006.01); C12P 19/12 (2006.01); C12P 19/18 (2006.01); C12P 19/24 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1051 (2013.01); C12N 9/92 (2013.01); C12P 19/12 (2013.01); C12P 19/18 (2013.01); C12P 19/24 (2013.01); C12Y 204/01007 (2013.01); C12Y 204/01064 (2013.01);
Abstract

The present invention relates to a method for producing trehalose, comprising the steps of mixing and reacting, in any order, (i) at least one alpha-phosphorylase capable of catalyzing the production of alpha-D-glucose 1-phosphate intermediate from a saccharide raw material, and from at least one phosphorus source; (ii) at least one trehalose phosphorylase capable of catalyzing the production of trehalose from an alpha-D-glucose 1-phosphate intermediate and a glucose substrate, wherein the trehalose phosphorylase is a trehalose phosphorylase variant with an amino acid sequence which differs from the amino acid sequence of a wild type trehalose phosphorylase in at least one amino acid position, (iii) at least one saccharide raw material which produces an alpha-D-glucose 1-phosphate intermediate and a co-product by catalytic action of the alpha-phosphorylase; and (iv) at least one phosphorus source selected from the group consisting of a phosphoric acids and an inorganic salt thereof.


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