The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 02, 2025

Filed:

Dec. 15, 2018
Applicants:

The Broad Institute, Inc., Cambridge, MA (US);

Massachusetts Institute of Technology, Cambridge, MA (US);

The Brigham and Women's Hospital, Inc., Boston, MA (US);

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Max Walt Shen, Cambridge, MA (US);

Jonathan Yee-Ting Hsu, Cambridge, MA (US);

Mandana Arbab, Cambridge, MA (US);

David K. Gifford, Cambridge, MA (US);

David R. Liu, Cambridge, MA (US);

Richard Irving Sherwood, Cambridge, MA (US);

Assignees:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G16B 20/30 (2019.01); A61K 31/7088 (2006.01); A61K 38/46 (2006.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); G16B 40/20 (2019.01);
U.S. Cl.
CPC ...
G16B 20/30 (2019.02); A61K 31/7088 (2013.01); A61K 38/465 (2013.01); C12N 9/22 (2013.01); C12N 15/1089 (2013.01); C12N 15/11 (2013.01); G16B 40/20 (2019.02); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01);
Abstract

The specification provides methods for introducing a desired genetic change in a nucleotide sequence using a double-strand break (DSB)-inducing genome editing system, the method comprising: identifying one or more available cut sites in a nucleotide sequence; analyzing the nucleotide sequence and available cut sites with a computational model to identify the optimal cut site for introducing the desired genetic change into the nucleotide sequence; and contacting the nucleotide sequence with a DSB-inducing genome editing system, thereby introducing the desired genetic change in the nucleotide sequence at the cut site.


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