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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12405265 B1

PDFFull Text
Date of Patent:
Sep. 02, 2025

Filed:

Jun. 14, 2021

Transient reporters and methods for base editing enrichment

Applicant:

Arizona Board of Regents on Behalf of Arizona State University, Scottsdale, AZ (US);

Inventors:

David Brafman, Phoenix, AZ (US);

Xiao Wang, Chandler, AZ (US);

Nicholas Brookhouser, Tempe, AZ (US);

Stefan Tekel, Tempe, AZ (US);

Kylie Standage-Beier, Phoenix, AZ (US);

Assignee:

ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US);

Attorney:

Quarles & Brady, LLP

Primary Examiner:

Kara D Johnson

Assistant Examiner:

Constantina E Stavrou

Int. Cl.
CPC ...
G01N 33/50 (2006.01); C07K 14/435 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5073 (2013.01); C07K 14/43595 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/907 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01);
Abstract

Provided herein are compositions and methods for real-time identification and isolation of base-edited cell populations. Also provided herein are methods for producing enriched isogenic lines of genetically modified cells, including base-edited human pluripotent stem cells. In particular, provided herein are methods utilizing transient expression of reporter proteins, the detectable signal of which is altered following base editing. Using the transient reporter with a base editor permits enrichment of isogenic populations of base-edited cells.

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Brafman, David. (2025). Transient reporters and methods for base editing enrichment (U.S. Patent No. 12405265). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/12405265

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