The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 02, 2025

Filed:

Jun. 06, 2018
Applicant:

Spark Therapeutics, Inc., Philadelphia, PA (US);

Inventors:

Guang Qu, Sicklerville, NJ (US);

Lin Lu, Wayne, PA (US);

Jesusa Josue-Almqvist, Havertown, PA (US);

John Fraser Wright, Princeton, NJ (US);

Assignee:

SPARK THERAPEUTICS, INC., Philadelphia, PA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/87 (2006.01); C12N 5/073 (2010.01);
U.S. Cl.
CPC ...
C12N 15/87 (2013.01); C12N 5/0603 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14151 (2013.01);
Abstract

Provided are compositions and methods of transducing/transfecting cells with a molecule, such as a nucleic acid (e.g., plasmid), at high efficiency. High efficiency transduced/transfected cells can, when transduced with a nucleic acid that encodes a protein or comprises a sequence that is transcribed into a transcript of interest, produce high amounts of protein and/or transcript. High efficiency transduced/transfected cells can, when transduced with plasmids comprising (i) nucleic acids encoding AAV packaging proteins and/or nucleic acids encoding helper proteins; and (ii) a transgene that encodes a protein or is transcribed into a transcript of interest; produce high amounts of recombinant rAAV vector.


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