The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 02, 2025
Filed:
Jun. 02, 2022
Evonik Operations Gmbh, Essen, DE;
Steliyana Dimitrova, Babenhausen, DE;
Patrik Stenner, Hanau, DE;
Silvia Blank-Shim, Aschaffenburg, DE;
Tobias Karl Hubert Müller, Limburg an der Lahn, DE;
EVONIK OPERATIONS GMBH, Essen, DE;
Abstract
The invention relates to a method of cell lysis for forming at least one pore in the cell wall and/or cell membrane of a cell in an aqueous medium for release of at least one intracellular target protein and/or lipid from the cell into the aqueous medium, including subjecting the cell with the intracellular target protein and/or lipid in the aqueous medium to an electrohydraulic comminution; and separating the target protein and/or lipid from the cell. The electrohydraulic comminution is brought about by a pulse current source. The cell in the aqueous medium is subjected to electrical impulse discharges between at least two electrodes of an underwater radio path of a container at a specific operating voltage, impulse energy, and pulse frequency, and at least one pore is formed in the cell wall and/or cell membrane.