The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 26, 2025
Filed:
Oct. 07, 2020
Access Medical Systems, Ltd., Palo Alto, CA (US);
Robert F. Zuk, Menlo Park, CA (US);
Pu Li, Los Altos, CA (US);
Jackie Shek Kei Chan, Mountain View, CA (US);
Indrani Chakraborty, Fremont, CA (US);
ACCESS MEDICAL SYSTEMS, LTD., Palo Alto, CA (US);
Abstract
The present invention is directed to biochemical assay methods, which re-use a hapten-immobilized test probe and reagents for quantitating an analyte or measuring kinetic binding in different samples, anywhere from about 3 to 20 times, while maintaining acceptable assay performance. The methods use a conjugate solution either comprising (i) an anti-hapten antibody and a capture antibody against an analyte, or (ii) an anti-hapten antibody and streptavidin, in each cycle and regenerate the hapten-coated test probe by dipping the test probe in an acidic solution having pH about 1-4, optionally further dipping in a DMSO solution, after the completion of each cycle of reaction. The robustness of the hapten-immobilized solid phase allows utilization of denaturation reagents for efficient elution of the immune complexes after each cycle without compromising the binding activity of the hapten on the solid phase.