The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 26, 2025
Filed:
Nov. 07, 2019
The Translational Genomics Research Institute, Phoenix, AZ (US);
Arizona Board of Regents on Behalf of Northern Arizona University, Flagstaff, AZ (US);
The Regents of the University of California, Oakland, CA (US);
Rebecca E. Colman, Flagstaff, AZ (US);
David M. Engelthaler, Flagstaff, AZ (US);
James M. Schupp, Flagstaff, AZ (US);
Paul Keim, Flagstaff, AZ (US);
David Smith, Flagstaff, AZ (US);
Antonino Catanzaro, San Diego, CA (US);
Timothy Rodwell, San Diego, CA (US);
THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE, Phoenix, AZ (US);
ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY, Flagstaff, AZ (US);
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US);
Abstract
The present invention provides a method of detecting a heteroresistant population of a pathogen in a sample, the method comprising: a) providing a sample comprising a population of a pathogen; b) extracting nucleic acids from the sample; c) amplifying a target locus of the genome of the pathogen in the extracted nucleic acids, wherein the target locus comprises at least one minor variant associated with drug resistance in the pathogen; d) consecutively sequencing both overlapping nucleic acid strands from a single DNA molecule amplified from the target locus on a Next Generation Sequencing (NGS) platform; e) applying an alignment algorithm to sequencing data from the overlapping nucleic acid strands; and f) performing an analysis of the aligned sequencing data to detect the at least one minor variant and heteroresistant population of the pathogen.