The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 26, 2025

Filed:

Jul. 10, 2015
Applicant:

Qiagen Sciences, Llc, Germantown, MD (US);

Inventors:

Yexun Wang, Ellicott City, MD (US);

Zhong Wu, Poolesville, MD (US);

Assignee:

QIAGEN Sciences, LLC, Germantown, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6869 (2013.01);
Abstract

The present disclosure provides oligonucleotides that comprise semi-random barcode sequences. Such oligonucleotides may be incorporated into reverse transcription primers, PCR primers, or portions of sequencing adapters in preparing sequencing libraries. The resulting sequencing libraries can be used for accurate sequencing, including DNA or RNA counting and mutation detection. Methods and kits for preparing sequencing adapters and sequencing libraries are also provided.


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