The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 26, 2025

Filed:

Aug. 28, 2019
Applicant:

Idemitsu Kosan Co., Ltd., Chiyoda-ku, JP;

Inventors:

Kazuyoshi Koike, Sodegaura, JP;

Eiji Takita, Sodegaura, JP;

Takeshi Matsui, Sodegaura, JP;

Assignee:

IDEMITSU KOSAN CO., LTD., Chiyoda-ku, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/16 (2006.01); C12P 21/02 (2006.01);
U.S. Cl.
CPC ...
C12N 9/16 (2013.01); C12P 21/02 (2013.01); C12Y 301/01003 (2013.01); C07K 2319/02 (2013.01); C07K 2319/21 (2013.01);
Abstract

By linking a peptide tag having the amino acid sequence X(PY)PZ to a protein of interest, and then expressing the protein, the protein of interest is efficiently accumulated in a soluble fraction, where P is proline; X is an amino acid sequence composed of 0 to 5 amino acids independently selected from R, G, S, K, T, L, N, Q, and M; Y is an amino acid sequence composed of 1 to 4 amino acids independently selected from R, G, K, T, L, N, Q, and M; q is an integer 1 to 10; and Z is an amino acid sequence composed of 0 to 10 amino acids independently selected from R, G, S, K, T, L, N, Q, and M; where the amino acid sequence of the peptide tag contains three or more Qs, Ms, Ls, Ns, and/or Ts in total.


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