The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 26, 2025

Filed:

Feb. 14, 2025
Applicant:

Allife Medicine (Beijing) Limited, Beijing, CN;

Inventors:

Shixin Gong, Beijing, CN;

Yuchun Gu, Beijing, CN;

Nan Li, Beijing, CN;

Qinqing Peng, Beijing, CN;

Lida Wu, Beijing, CN;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/0786 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0645 (2013.01); C12N 2500/02 (2013.01); C12N 2500/25 (2013.01); C12N 2500/32 (2013.01); C12N 2500/38 (2013.01); C12N 2500/44 (2013.01); C12N 2500/50 (2013.01); C12N 2501/052 (2013.01); C12N 2501/115 (2013.01); C12N 2501/125 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/165 (2013.01); C12N 2501/22 (2013.01); C12N 2501/2303 (2013.01); C12N 2501/2305 (2013.01); C12N 2501/2313 (2013.01); C12N 2501/24 (2013.01); C12N 2501/727 (2013.01); C12N 2506/45 (2013.01); C12N 2523/00 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01);
Abstract

A medium combination and a method for inducing iPSC differentiation to obtain macrophages and use thereof are provided. The medium combination includes a first stage medium to a sixth stage medium. The first stage medium is an E8 complete medium containing a ROCK pathway inhibitor and polyvinyl alcohol, the second stage medium is an E8 complete medium containing a GSK-3β inhibitor, the third stage medium includes an M1 medium and an M2 medium, the fourth stage medium is an M3 medium, the fifth stage medium is an M4 medium, and the sixth stage medium is an M5 medium.


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