The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 26, 2025

Filed:

Apr. 09, 2019
Applicant:

Massachusetts Institute of Technology, Cambridge, MA (US);

Inventors:

Roger Dale Kamm, Cambridge, MA (US);

Tatsuya Osaki, Cambridge, MA (US);

Sebastien Guy Marcel Uzel, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 3/06 (2006.01); A61K 31/436 (2006.01); A61K 31/4706 (2006.01); A61K 31/496 (2006.01); A61K 45/06 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12N 5/0793 (2010.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12M 23/16 (2013.01); A61K 31/436 (2013.01); A61K 31/4706 (2013.01); A61K 31/496 (2013.01); A61K 45/06 (2013.01); C12M 23/04 (2013.01); C12M 23/34 (2013.01); C12M 33/04 (2013.01); C12M 41/40 (2013.01); C12M 41/48 (2013.01); C12N 5/0619 (2013.01); G01N 33/5058 (2013.01);
Abstract

Microfluidic devices with neuronal cells, muscle cells, and optionally other cell types co-cultured therein are provided. Typically one or more the cells has a mutation that contributes to or causes a neuronal or muscular disease or disorder. For example, in some embodiments, one or more of the cultured cells are derived from a subject with a neuronal or muscular disease or disorder. The microfluidic device can facilitate formation of a 3D motor unit and a neuromuscular junction in vitro, and be used to monitor the molecular, biochemical, cellular, and morphological differences in the formation of such structures by healthy and diseased cells, and for testing compounds, dosages of compounds, dosing regimes, and combinations thereof, that may improve or worsen their formation. An exemplary combination drug therapy identified in this way is also provided.


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