Full-ocean-depth fidelity enzymological measurement device for microbial extracellular enzymes

Abstract

A full-ocean-depth fidelity enzymological measurement device for microbial extracellular enzyme is provided, comprising a pressure-maintaining sampling bottle, pressure-maintaining transfer equipment, a pressure-maintaining enzymological reactor, and heat preservation equipment and enzyme activity detection equipment. The pressure-maintaining enzymological reactor comprises a barrel body, a plug, polytetrafluoroethylene gaskets, an O-ring, a piston, a high-pressure straight-through valve, and a high-pressure connector. The pressure-maintaining enzymological reactor is in a closed barrel body shape and is internally provided with the piston, and the plug and the valve are arranged at each of two ends of the pressure-maintaining enzymological reactor; and the valve is connected to the pressure-maintaining transfer equipment through the high-pressure connector. According to the full-ocean-depth fidelity enzymological measurement device provided by the present disclosure, full-ocean-depth (-m) sample pressure-maintaining sampling and transferring can be achieved; and sample collection, transferring and enzymological reaction can be conducted under in-situ pressure and temperature conditions.