The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 26, 2025

Filed:

Mar. 08, 2021
Applicants:

Kabushiki Kaisha Toshiba, Tokyo, JP;

Shinshu University, Nagano, JP;

Inventors:

Eiichi Akahoshi, Tokyo, JP;

Mitsuko Ishihara, Tokyo, JP;

Yozo Nakazawa, Matsumoto, JP;

Daisuke Morita, Matsumoto, JP;

Assignees:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 14/705 (2006.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/42 (2025.01); C07K 16/28 (2006.01); C12N 5/0783 (2010.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
C07K 14/705 (2013.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/4211 (2025.01); C07K 16/2809 (2013.01); C07K 16/2818 (2013.01); C12N 5/0636 (2013.01); C12N 15/113 (2013.01); C12N 2501/998 (2013.01); C12N 2510/00 (2013.01);
Abstract

According to one embodiment, there is provided a method of producing a gene modification T cell (CAR-T cells) which expresses a chimeric antigen receptor (CAR). This method includes stimulating a cell population containing a T cell with an antibody which activates the T cell, bringing the cell population into contact with a nucleic acid-introducing carrier, wherein the nucleic acid-introducing carrier containing a lipid particle, a first nucleic acid and containing a CAR gene, and a second nucleic acid containing a transposase gene encapsulated in the lipid particle, and culturing the cell population after the contacting.


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