The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 19, 2025

Filed:

Mar. 30, 2020
Applicant:

National Research Council of Canada, Ottawa, CA;

Inventors:

Traian Sulea, Kirkland, CA;

Jason Baardsnes, Montreal, CA;

Christopher R. Corbeil, Dollard-des-Ormeaux, CA;

Maria L. Jaramillo, Beaconsfield, CA;

Enrico O. Purisima, Pierrefonds, CA;

John C. Zwaagstra, Laval, CA;

Nazanin Larijani Rohani, Montreal, CA;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/574 (2006.01); A61K 47/68 (2017.01); A61P 35/00 (2006.01); C07K 16/32 (2006.01);
U.S. Cl.
CPC ...
G01N 33/57415 (2013.01); A61K 47/68033 (2023.08); A61K 47/6855 (2017.08); C07K 16/32 (2013.01); A61P 35/00 (2018.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/35 (2013.01); C07K 2317/55 (2013.01); C07K 2317/77 (2013.01); C07K 2317/92 (2013.01); C07K 2319/33 (2013.01);
Abstract

The present invention is directed to anti-Her2 antibodies and fragments thereof, compositions, and uses thereof. The antibodies and fragments thereof bind to Her2-expressing cells in a pH-dependent manner, with low affinity at the physiological pH typical of normal cells and tissues, and with high affinity at the slightly acidic pH characteristic to solid tumors. Furthermore, the antibodies inhibit the growth of tumor spheroids at a level comparable to that of the benchmark anti-Her2 antibody Herceptin at acidic pH, whereas these effects were significantly reduced at physiological pH. This pH selectivity of cellular growth inhibition is maintained upon antibody conjugation to cytotoxic drugs. The invention also includes methods of treating solid tumors, methods of detecting solid tumors, and methods of pH-dependent capturing of the Her-2 ectodomain.


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