The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 19, 2025

Filed:

Mar. 03, 2023
Applicant:

Toagosei Co., Ltd., Tokyo, JP;

Inventors:

Nahoko Baileykobayashi, Tsukuba, JP;

Tetsuhiko Yoshida, Tsukuba, JP;

Assignee:

TOAGOSEI CO., LTD., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/50 (2006.01); C07K 7/08 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5008 (2013.01); C07K 7/08 (2013.01);
Abstract

The herein disclosed method for introducing a foreign substance of interest from outside a eukaryotic cell into at least a cytoplasm of the cell, comprises: (1) a step of preparing a foreign substance introduction construct that has a carrier peptide fragment comprising either of the amino acid sequences: KKRTLRKSNRKKRWPC(SEQ ID NO: 1) and KKRTLRKKKRKKRWPC(SEQ ID NO: 2), and that has the foreign substance of interest bonded to an N-terminal side and/or C-terminal side of the carrier peptide fragment; (2) a step of supplying the foreign substance introduction construct into a sample that contains a target eukaryotic cell; and (3) a step of incubating the sample into which the foreign substance introduction construct has been supplied, to thereby introduce the construct into the eukaryotic cell in the sample.


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