The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 19, 2025

Filed:

Aug. 02, 2024
Applicant:

Shennon Biotechnologies Inc., San Francisco, CA (US);

Inventors:

Yih Yang Chen, San Francisco, CA (US);

Kaveh Milaninia, San Jose, CA (US);

Li Sun, San Francisco, CA (US);

Assignee:

Shennon Biotechnologies Inc., San Francisco, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); B01F 23/41 (2022.01); C12N 5/00 (2006.01); C12N 11/04 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0012 (2013.01); B01F 23/41 (2022.01); B01L 3/502792 (2013.01); C12N 11/04 (2013.01); B01L 2400/02 (2013.01);
Abstract

Disclosed are methods and microfluidic devices for successfully co-encapsulating two or more cells in a high-throughput, high efficiency manner. Cells are organized into two or more ordered streams flowing through separate microchannels of the microfluidic device. Cells in ordered streams are sufficiently spaced such that at a junction of the microfluidic device, single droplets are generated that include exactly one cell from the first ordered stream of cells and at least one cell from the second ordered stream of cells. Single droplets including two or more cells are useful for performing assays (e.g., high throughput cell-cell interaction assays).


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